期刊刊名：南台學報 　 卷期：38卷1期

篇名出版日期：2013年3月1日

作者：許德發,湯惠平,許照紅,黃楷能,Te-Fa Hsu, Huei-Ping Tang, Chao-Hung Hsu, Jeng-Chai Huang

語言：English

關鍵字：ATP, ADP, AMP, Adenosine, HPLC, Energy Charge,腺核苷三磷酸,腺核苷二磷酸,腺核苷單磷酸,高壓液態層析儀,能量積

被點閱次數：5次

閱讀時間：446sec

摘要：
Most reported methods for HPLC have shown chromatography of biological adenosines in clean buffers, indicating a defect in the method used. In the present research an amine column equipped-HPLC system together with a fluorescent derivatization of 1,N⁶-ethenopurines using chloroacetaldehyde, and conditioned at pH 6.0 to decrease adenosines degradation, it were tested for the purpose of biological energy charge analysis in the liver. The fluorescent derivatives of ATP, adenosine metabolites, or its analogues taken from a rat’s liver were prepared, and specifically resolved using HPLC. The amine column seems to have both the stationary merits of the reverse phase and ion exchange characteristics. This HPLC system enables us to analyze the energy charge in the liver that represents mitochondria functions. The achievement of this study may help us to interpret the relationships of energy charge on hepatocytes and its apoptosis.

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