期刊刊名：南台學報 　 卷期：38卷1期

篇名出版日期：2013年3月1日

作者：陳勝咸,Sheng-Hsien Chen

語言：English

關鍵字：Human Umbilical Cord-derived Mesenchymal Stem Cell, Heatstroke, Inflammation, Coaggulation, Cerebral Ischemia and Damage,人類臍帶間質幹細胞,熱中風,發炎,凝血病變,大腦缺血性損傷

被點閱次數：4次

閱讀時間：24sec

摘要：
Heatstroke is a lethal disease characterized by hyperpyrexia. Hypotension, hepatic and renal failure, entering a hypercoagulable state, systemic inflammation and cerebral ischemia, and all the injury and dysfunction accompanying these conditions. Our previous study [Do you mean in a paper you published earlier? If you mean the present research, say so, and not “previous”] showed that human umbilical cord blood cells (hUCBC) derived from hematopoietic stem cell (hUCBHSC) transplantation can resuscitate rats in which heatstroke had been experimentally induced. Although it has been shown that mesenchymal stem cells derived from human umbilical cord can be used to rescue mice from potentially lethal heatstroke, the hypothesis here proposed is that it also improves survival during heatstroke by attenuating multiorgan dysfunction. The experimental animals consist of male, 8-wk-old ICR mice. The animals were randomly divided into three groups: a normothermic control group, a Saline vehicle-treated heaststroke group, and a hUCMSC vehicle-treated heaststroke group. The normothermic control group were left at room temperature. The other two groups were subjected to the animal heatstroke model of whole body hyperthermia (WBH) at 41.5℃ for 1 hour and then allowed to recover at room temperature. Mice that survived 4 days of WBH were classified as survivors. Concurrently with this research, immunohistochemical staining was employed to assess cell damage and apoptosis. It was expected that hUCMSCs would attenuates heatstroke mortality. The main results show that hUCMSC vehicle-treated mice all had significantly (1)attenuated heatstroke-induced mortality, (2)diminished neuronal brain damage, and (3)reduced numbers of apoptotic cells in the hypothalamus. In conclusion, MSCs can protect the mice from heatstroke-induced neuronal brain damage and attenuatee their mortality rate.

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